Hi,
On Sun, Feb 6, 2011 at 8:32 PM, crystallogrphy
Hi, I am refining a hexamer model using low resolution data (4.7A). I want to use a high resolution model (2.4A) which is a dimer as the reference model. The sequence identity is 100%.
Does anyone know how to select the residue range from the reference model for my refinement?
In this case, it will depend on how you want to assign the reference model to the working model. Do you want to assign one chain from the reference model as the reference for each protein in the working hexamer, or do you want to assign the dimer in the reference model to each pair of proteins in the working model? For you first case, you'll want something like this: refinement { main { reference_model_restraints=True } reference_model.file=reference.pdb reference_model.reference_group { reference = chain A selection = chain A } reference_model.reference_group { reference = chain A selection = chain B } reference_model.reference_group { reference = chain A selection = chain B } etc... } If you want to assign the reference pairings in a different way, use a similar construction but with different selections for the reference_model.reference_group entries. You can read more about this in the phenix documentation: http://www.phenix-online.org/documentation/refinement.htm#anch28 If you have further questions please let me know. Thanks, Jeff
Thanks!
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