Re: [phenixbb] Phenix Phaser Question

Dear Hyun Jeong, It’s worrying if you get highly ambiguous solutions in Phaser, because most of the time a correct solution is unique. Could you email me the log file from the job that found 18 possible solutions (preferably off-list)? That might give some ideas about what is going on. Best wishes, Randy Read

On 11 Jul 2023, at 05:29, hyun4867 wrote:

Dear, Everyone

Hi all, I am Hyun Jeong. I want to ask you about phenix phaser problem.

I obtain diffraction pattern of crystal that is complex. I tried to solve the phase based on the Apo structure i previoulsy solved.

Using full-featured phaser, i obtain 18 Phaser solution. This is really strange number. But, the map obtained 18 Phaser solution is fitted in electron density map.

May I proceed with processing using the discovered electron map with 18 Phasers? Is it necessary to redo the scaling?

I appreciate your request for advice.

Best regards, Hyun Jeong _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb Unsubscribe: [email protected]

----- Randy J. Read Department of Haematology, University of Cambridge Cambridge Institute for Medical Research Tel: +44 1223 336500 The Keith Peters Building Hills Road E-mail: [email protected] Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk