Dear all, I was re-running some former refinements (done with 1.13-2998) with 1.14-3374. Rwork and Refree increased significantly and stereochemistry got slightly worse. Any idea? Am I ignorant to something? Below are just some lines of the summaries. Thanks a lot and best regards, Guenter 1.13 Ramachandran outliers = 0.00 % favored = 99.09 % Rotamer outliers = 1.30 % C-beta deviations = 0 Clashscore = 3.47 RMS(bonds) = 0.0096 RMS(angles) = 0.96 MolProbity score = 1.22 Resolution = 1.90 R-work = 0.1770 R-free = 0.2084 1.14 Ramachandran outliers = 0.73 % favored = 98.73 % Rotamer outliers = 0.56 % C-beta deviations = 1 Clashscore = 6.23 RMS(bonds) = 0.0118 RMS(angles) = 1.25 MolProbity score = 1.34 Resolution = 1.90 R-work = 0.1902 R-free = 0.2336