I am trying to solve a structure by molecular replacement  in phaser using 2 model fragments. I think I may have placed the 1st fragment correctly. I don&#39;t quite understand how to best proceed with a fixed ensemble searching for the second fragment in phaser-MR. If I select &quot;use partial solution from previous job&quot;, do I still need to read in the coordinates of that previous solution for one ensemble? And If I do, should I read in the starting coordinates or the placed output from this 1st molecular replacement?<br>
<br>The second question is about how to relax the restraints fr the packing. In the &quot;other settings&quot; sub window is a section for packing. Could someone explain to me what the different options in this packing section mean? Which of them should I change to allow for more close contacts?<br>
<br>Thanks<br><br>Ursula<br clear="all"><br>-- <br>Ursula Schulze-Gahmen, Ph.D.<br>Assistant Researcher<br>UC Berkeley, QB3<br>356 Stanley Hall #3220<br>Berkeley, CA 94720-3220<br>