We recently determined a protein structure by molecular replacement and the statistics are looking good, however, the average B-factor is unusually high, and the Wilson B-factor is high too.  A quick research on the subject suggested that high B-factors are acceptable as long as one has excluded and double-checked for other crystallographic problems. Which problems should I check for and what is your opinion on the high B-factor? Is there anything that could be done to lower it? 

Resolution: 2.5 A 

Space group P3121, 60.0 60.0 142.6 90 90 120

R work 24.6%

R free 26.4%

Wilson B-factor 73.2

Average B-factor 99.5

Thank you for your advice,

Best, Sozanne.