Hi Vincent,

I am trying to superpose a CryoEM map onto a Xray map.
I tried the "superpose maps" under the map section of phenix 1.14 but it only takes "reflections" and not the .mrc format  of the CryoEM map.
should I transform the CryoEM map into structure factors using "Map to structure factors" or is there a better way to do this?

you can try.. Yes, convert both maps into Fourier space equivalents and feed into the program. Note, you need  two models that fit two maps. The way it works is that it superposes models first, then applies corresponding operators (that superpose models) to maps. I can't remember anyone asking this questions before on this list or myself directly.

Share your experience!

Good luck,
Pavel