Not personally. Two people in the lab have done it and are significantly benefiting (and I cannot volunteer their datasets, since the structures are not finished or published yet). However, you can probably get from the pdb database the Delabarre and Brunger data for p97/VCP at 4.4 A (PDB ID: 1YQ0) as explained in Acta Cryst D 62 p. 923. It is implemented in CNS following this paper. Among our lab people the two cases where it really worked were both 3.5 A datasets with experimental phasing (as the Brunger papers also point out to), and in cases without experimental phasing, I have not seen much improvement. The magical values in one case was -100 A^2, and in the other -50 to -100 (For the published Brunger data, it was -120). Thank you, Engin Pavel Afonine wrote:
Hi Engin,
yes, it is in the list of things to do.
Do you have a specific example of where B-factor sharpening really helps (data, model, and the magic value for B-sharp) that I can use as a reference?
Pavel.
On 1/23/2009 1:43 PM, Engin Ozkan wrote:
I know this has been asked before, and there are ways around to get this done in Autosol or Xtriage, but a sharpening B-factor for low resolution maps would be a very nice addition to phenix.refine. Are there any plans to add a parameter in phenix.refine for this?
Best,
Engin _______________________________________________ phenixbb mailing list [email protected] http://www.phenix-online.org/mailman/listinfo/phenixbb