Hi, Is there a chance of backsoaking even when the protein has been expressed as a selenomethionine derivative? I just overexpressed the protein by shutting down the methionine biosynthesis in E.coli and incorporated SeMet instead of methionine. The protein has three methionine and is 26kDa Shya
Did you "backsoak" the crystal? Wouldn't heavy atom in the mother liquor give a fluorescence peak even if none of it bound? Ed
[email protected] wrote:
Hi, I had similar problems recently, I did a wavelength scan of my crystal at the synchrotron (selenium edge) and collected one dataset at the peak wavelength.The scan gives me a distinct peak. However when I processed the data and evaluated in xtriage it says that there is no anomolous signal. I am unable to explain this? any advice? Shya
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