18 Apr
2014
18 Apr
'14
3:01 p.m.
Hi Xiang, can you please send me data and model files so I can have a closer look? If as you say you used base-pair restraints they should not flip, and if they do this tells me there is something wrong with base-pair restraints, and so to investigate this I'd like to see the files. Thanks, Pavel On 4/18/14, 6:53 AM, 李翔 wrote:
Hi,
I am working my low resolution data (6A) by MR and met a problem about DNA basepairing during refinement. I think so far everything looks OK except the bases flipped during the refinement. I added basepairing restraint for all bases, however, seems not help too much. Does anyone have experience on that?
Sincerely, Xiang