A question from my collaborators: what is the best way to get phenix to handle a GTP as nt #1 of an RNA chain?   That is, what is the best way to specify a covalent bond with the proper geometry between the 3’ O of GTP1 and the 5’ P of nt #2?

Thanks in advance,

  Phoebe

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Phoebe A. Rice

Dept. of Biochem & Mol. Biol. and

  Committee on Microbiology

https://voices.uchicago.edu/phoebericelab/