I am refining a structure containing SeMet with data collected at peak wavelength. Provided I give the f' and f'', will phenix.refine calculate F+ and F- structure factors and use the provided FOBS+ and FOBS- ? CNS does this. Refmac only uses f' and I get >6 sigma Fo-Fc peaks on the Se.

Thanks for your help.

_________________________________

Mark A. Saper, Ph.D.

Associate Professor of Biological Chemistry

Biophysics Research Division, University of Michigan

930 N University Ave

Ann Arbor MI 48109-1055 U.S.A.

[email protected] (734) 764-3353 fax (734) 764-3323

http://www.biochem.med.umich.edu/biochem/research/profiles/saper.html