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5 Dec
2007
5 Dec
'07
4:12 p.m.
I have used 3 programs to refine RNA structures recently: CNS, Refmac and Phenix. Of these, only CNS has the temerity to enforce what it thinks is a correct sugar pucker to the extent that it will pull atoms out of the electron density. Refmac and Phenix both do a much better job of refining non-canonical sugar puckers in RNA in my experience. Joe Krahn wrote:
I see that phenix is using the same kind of simplistic statistical restraints as CCP4. Are there plans to improve the geometry restraints once the rest of phenix is stable?