Hi Mohamed, perhaps you can copy some images from here: http://phenix-online.org/presentations/latest/2012_afonine_ecm27-final.pdf and also here http://journals.iucr.org/d/issues/2010/11/00/dz5209/index.html Or simply - get data and model from PDB phenix.fetch_pdb pdb_code --mtz - compute maps phenix.maps model.pdb data.mtz or phenix.maps model.pdb data.mtz if it is neutron data set. You may want to remove H so that you can see them in residual maps: phenix.reduce -trim model_with_h.pdb > model_no_h.pdb - use graphical program of your choice to make a picture (I use Pymol). Examples above use Phenix command line. All the same can be done using the GUI. Pavel On 4/17/15 2:58 PM, mohamed noor wrote:
Dear all
I will be presenting about protein crystallography to a group of PhD-level physicists and need some images from the community.
Would anyone have high-quality images of density maps that show hydrogen atoms at 1. high and ultra-high X-ray resolution (say, about 1.2 A to about 0.5 A) 2. low or medium X-ray resolution (where H atoms are not visible) compared with neutron data (with H). Ideally these images should be from the same protein so that I can show the power of complementary beam source for the diffraction.
A side question - what is the community's preference to get deuterated samples? Is it crystal soaking or growing E. coli in deuterium?
All contributions will be credited. Thanks.
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