Does anyone know how to generate a custom NCS definition file that AutoBuild will read from the refinement format? The parameter is input_ncs_file =

I used phenix.simple_ncs_from_pdb to generate 3 ncs format files. But I could not figure out how to make the program use my definitions. In any case simple_ncs_from_pdb outputs 3 formats in ./phenix/tmp:

simple_ncs_from_pdb.ncs
simple_ncs_from_pdb.ncs_spec
simple_ncs_from_pdb.resolve

I tried the last one, but it has no residue or chain specification. Should I use the .ncs_spec? The first one is for phenix.refine.

Thanks, Mark

_________________________________

Mark A. Saper, Ph.D.

Associate Professor of Biological Chemistry

University of Michigan

Biophysics, 3040 Chemistry Building

930 N University Ave

Ann Arbor MI 48109-1055 U.S.A.

[email protected] phone (734) 764-3353 fax (734) 764-3323

http://www.biochem.med.umich.edu/?q=saper http://www.strucbio.umich.edu/