Dear Colleagues,We hereby announce a 4-day workshop on “Sample Preparation for Single Particle Analysis by Cryo-Electron Microscopy” [1]. The course is funded by Instruct-ERIC and will be held at the Iberian Nanotechnology Laboratory (Braga, Portugal), between the 7th and 10th of October this year.The Workshop is aimed primarily at Ph.D students and young Post-Doctoral researchers, is set to provide in-depth training on sample preparation for single particle analysis (SPA) by cryo-electron microscopy (cryo-EM), and is hosted by a team of experts from ITQB NOVA [2], INL [3], CNB–CSIC [4], i3S [5], and SciLifeLab [6].Participants will engage in a comprehensive course featuring a blend of lectures and hands-on tutorials. The lectures will delve into the principles of SPA, covering theoretical and practical aspects of preparing biological samples for negative staining, quality assessment and data collection. The workshop will highlight recent advancements in protein production and stabilization, as well as the latest technological innovations in grid preparation and sample manipulation.Attendees are encouraged to bring their samples, providing a unique opportunity to apply newly acquired skills to their specific research needs. Practical sessions will offer hands-on experience with vitrification using a plunge-freezing device and optimizing grids for sample screening with a cryo-transmission electron microscope (TEM).Participants will be limited to 12 to allow a closer interaction between the participants and the tutors. Candidate selection will be based on a short CV, a motivation letter from the Applicant, and a recommendation letter from the Supervisor.Registration deadline is August 19, 2024, and can be done at the following link:https://www.events.inl.int/instruct-eric-workshopRegistration fee is 250Eur for Academics and 500Eur for Non-Academic, and includes accommodation, lunches, coffee-breaks and social dinner. Please feel free to contact us if you have any question.On behalf of the organizing team, hoping to see you in Braga,Jose Artur Brito – ITQB NOVA