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26 Jun
2013
26 Jun
'13
3:20 p.m.
On Wed, Jun 26, 2013 at 2:05 AM, Mark J van Raaij
Can't you just refine the poly-Ala model against the 1.5A native data? (Perhaps after rigid body refinement or even molecular replacement if the data is too non-isomorphous). And then run Autobuild with these phases?
If the model only contains around 50% of residues, and no sidechains, the phases from refinement are going to be fairly poor. I've never had much luck trying to autobuild starting such low completeness, even with very good data. -Nat