Hi everyone, I have a dataset in P21221. The cell content analysis shows that there should be 5 monomers in asu. The gel filtration profile of this protein shows that it should form a tetramer. I have a simulated structure as searching model. I try to use the whole model or backbone model, and different resolution cutoff. When the whole model is used, the llg of solution is negative. When the backbone model is used, the llg is about 160 when searching for 4 monomers and tfz is 7.4. But the following autobuild process could not lower rfactor than 50%. I notice online that it might be useful to run self rotation function first to find ncs operators before doing mr. Is this true and how can i do this? Thank you in advance! Fengyun