Hi,<br><br>I have a couple of questions regarding low res structures in Phenix (about 3.5 angstroms res).&nbsp; <br><br>1.&nbsp; Can Phenix do B-factor sharpening for improved maps?&nbsp; If not what program do people usually use for this?
<br><br>2.&nbsp; How can one optimize Ksol and Rprobe (bulk solvent model) in Phenix?<br><br>3.&nbsp; Are there any other suggestions for dealing with low resolution structures in Phenix?<br><br>Thanks,<br>John Bruning<br>