Re: [phenixbb] Improvement of low resolution MR phases

29 Jun 2021

      Dear Tommi,
I have thought before about this kind of case, of 5 to 6 Angstrom resolution diffraction, namely that the molecular transform must increase again at higher resolution, say 2 1/2 Angstrom, at which therefore the measurable intensity would increase enough to maybe be above background. 
To check this go back to your diffraction images and integrate to, say, 2Angstrom. Does the  show the increase at around 2 1/2 Angstrom resolution?
Obviously if you do have those higher resolution data measurable then matters improve all round.
Greetings,
John 

Emeritus Professor John R Helliwell DSc

...
On 28 Jun 2021, at 12:56, Kajander, Tommi A  wrote:
 Hello all,
I was wondering what would be current best protocols for trying to improve maps/phases for low resolution 
MR / refinement solutions (5-6 Å resolution).
High solvent content of course, dimeric 2:2 protein complex so some NCS averaging and density modification
I assume might help (related structures known, currently maps very blobby, clear solution but I think should be able to do better...).
Also, refinement suggestions welcome.
Best route in phenix? So far worked initially with ccp4. Other suggestions also welcome.
Thanks!
Tommi
Tommi Kajander, Ph.D. 
Institute of Biotechnology
University of Helsinki
Viikinkaari 1 (P.O. Box 65)
00014 Helsinki, Finland
p. +358-50-4480991
http://www.helsinki.fi/kajanderlab
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