[phenixbb] questions on refinement

Hi  all How many cycles are required to remove the Rfree bias for different R-free data set. I usually do annealing at CNS. For the different Rfree set,   I used to give 5 or 10 cycles of refinement, but Rfree varies between 2% between the cycles. I like to know that just adp refinement or annealing_torsion is required for different R-free set? How many cycles will be sufficient to remove rfree bias? Thanks Ram