Hi all,
Can you please advise once we fit the map and open in coot. What is next step to do in coot. I tried refmac but that required ccp4 file which doesn't have. Please some one can help in elaborating and sharing any tutorial would be helpful. I'm new bie and first time I am trying model building. Any help would be really appreciated. Thank you so much
Best wishes
Tim
Hello Iram,The general workflow for Cryo-EM is described here:It looks like you are ready to refine your model, so the tool for it is phenix.real_space_refine:that also has a video tutorial.Let us know if you have any questions.Best regards,Oleg Sobolev._______________________________________________On Thu, Dec 12, 2024 at 5:54 PM iram aziz <iram.sbs@pu.edu.pk> wrote:Hello All._______________________________________________I got a nice 2.45 Angstrom map from cryo em data. I checked handedness, map model fitting on the chimera and opened in the coot. It looks nice. Can you please give me suggestions about the Phenix refinement steps.ThanksIram
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