![](https://secure.gravatar.com/avatar/f3de86147d439720e7eb4802800a75be.jpg?s=120&d=mm&r=g)
9 Mar
2011
9 Mar
'11
4:17 p.m.
Hello everyone, I have this strucuture I solved by Molecular replacement. It is a single mutant of previously solved enzyme. I started refining it and fit the ligand in a way that gives me the experimental stereochemistry. The final map 2mFo-2DFc looks great. After further examination I noticed, I have a carbonyl that does not seem to be bound to anything !!! I started looking at my average kick and SA composit omit-map prior to modeling in the ligand and now I am really lost. Could anyone help? Thank you