It may mean a variety of things, such as:

- troubled refinement, incorrect target weights for instance;
- suboptimal model parametrization or/and optimization method choice;
- incorrect test/work flag use in refinement;
- this sometimes happens if you refine against ultra-high resolution (higher than say 0.7A).

If you send more information such as at least refinement log and eff files then it may be possible to tell more.

Pavel

On 3/30/15 8:01 AM, Smith Liu wrote:
Dear All,
 
If in a Phenix.refine proces, the Rfree continuously lower than Rwork, what does it mean?
 
Smith