What about trying heavy atom phasing? Like introducing Se-Mets in your protein for MAD data collection. Sometimes you can waste plenty of time trying molecular replacement and the solution never comes out. particularly when you have no sequence homology... Fred. shuchi moni wrote:
i have one data of 3 A of a unknown protein don't have sequence homlogous pdb known . but showing highly coserved fold.
space group is p622 and showing hexamer in assymmetric unit . in molecuar replacement with a single monomer when i m searching 6 copies lots of clashes are coming. with single copy serach i m getting fully fit template in density but unable to search density for others ensembles. how should i proceed for this
regards vandana kukshal ------------------------------------------------------------------------
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