Dear user,
                         I have problem in finding the phase for the flexible DNA binding domain of LTTR protein. The unit cell parameters are ,Unit Cell: 46.92 105.30 47.75 90.00 103.26 90.00, and space-Group P 1 21 1. Molecular weight of protein is 36000Da. When i am running phaser it showing that
Most probable MW of protein in asu for resolution = 49714.8. Could you please guide me how to set the proper setting for finding the correct rotation function and translation function setting to get the phases for the full length protein. I m getting the phases for the ligand binding domain but not for the DNA binding domain. Could you please suggest me some tricks which would work to get the phase. Thanks a lot in advance.
Appu