Dear Nathaniel,

Thanks for your reply. So from your answer, I feel that maybe it's not worth to spend much time to resolve the structure through MR based on current dataset? Even I can correctly place the domainB, it's still hard to resolve the intact protein structure without other phasing information, is that right?

Thanks again and best regards!

Zhihong

I confess I haven't had to solve a structure like this myself - just seen a bunch of datasets where similar attempts didn't work - but it's hard to see how it can be solved without experimental phasing information (SeMet, etc.). The good news is that if you can get a correct MR solution for domainB, you can use MR-SAD to find heavy atom sites and solve the structure, which should be easier than starting from no phases at all.

-Nat