Re: [phenixbb] how to apply phi/psi helical restraints

I downloaded the latest build (dev-833), but could not see a Ramachandran restraints option under Phenix.refine GUI /all parameters/atom selections. Am I missing something? Thanks. Daqi On 7/29/2011 1:53 PM, Nathaniel Echols wrote:

On Fri, Jul 29, 2011 at 10:45 AM, Pavel Afonine wrote:

...

I can't see why doing both: defining custom bonds between N-O pairs that stabilize the helical region and using Ramachandran plot restraints would not work. By default Ramachandran restraints are applied to all residues - this is very risky, especially when the goal is to keep secondary structure from unraveling. It would be prudent to define the parameter rama_selection (in the GUI, "Atom selection for Ramachandran restraints") to include only the helical regions.

What is the resolution of this structure?

-Nat _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb