I've done just that with GTP, so with minor changes, this should work: geometry_restraints.edits { bond { action = *add delete change atom_selection_1 = "chain A and resid 1 and name O3\\*" atom_selection_2 = "chain A and resid 2 and name P" symmetry_operation = None distance_ideal = 1.59 sigma = 0.01 } angle { action = *add delete change atom_selection_1 = "chain A and resid 1 and name C3\\*" atom_selection_2 = "chain A and resid 1 and name O3\\*" atom_selection_3 = "chain A and resid 2 and name P" angle_ideal = 120 sigma = 1 } } Francis E Reyes wrote:
All
I need to attach a 5' modification, a diphosphate guanosine to my RNA. I figure GDP (from the monomer library) has everything I need. However, what needs to be done to make a connection with the next nucleotide in the chain (in both phenix.refine and coot) ?
Thanks
FR
--------------------------------------------- Francis Reyes M.Sc. 215 UCB University of Colorado at Boulder
gpg --keyserver pgp.mit.edu --recv-keys 67BA8D5D
8AE2 F2F4 90F7 9640 28BC 686F 78FD 6669 67BA 8D5D
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