Hi ,
I am solving a structure using selenium SAD using autosol. Autosol gave me nice solution but many loop and side chain density is missing.
autosol gave me many different output MTZ files .
for building further in coot which MTZ file i should use and for refinement in phenix refinement which MTZ file i should use . I tried doing refinement with .sca file it gave me increase Rfree afte single cycle of refinement .
thanks for your suggestions.
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Vandna Kukshal
Postdoctral Research Associate
Dept. Biochemistry and Molecular Biophysics
Washington University School of Medicine
660 S. Euclid, Campus Box 8231
St. Louis, MO 63110