Hi Saif, If I understand you correctly, you can specify the search model with 8 polypeptides as one PDB file and all 8 sequences (separated by blank lines or lines starting with ">" in one sequence file. Also component 1 can be all 8 polypeptides and copies=1. All the best, Tom T
Hello,
I have a membrane protein complex made up of 8 different polypeptides present in two copies each (16mer). The asymmetric unit is the octamer, as determined from previous crystallographic studies. To carry out molecular replacement with the phases from the published structure in AutoMR, should I specify the coordinates of the search model and its overall sequence identity as one file or should I break it down for each subunit ? Also, for the asymmetric unit contents, should I specify the sequence or mass of each subunit separately (as component 1, 2 and so on) keeping the number of copies fixed at 1 ? There is no NCS.
Thanks. -- S. Saif Hasan
Graduate Student Department of Biological Sciences Hockmeyer Hall of Structural Biology Purdue University 240 S. Martin Jischke Drive West Lafayette, IN 47907
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