Dear all,
I'm working on a 18kD protein, the secondary structure prediction says most
of the structure is beta sheets, trying to solve the structure with SAD.
Heavy atom soaking gives several datasets with I, W, Au, range from
2.7~3.7A, however, the anomalous signal is pretty weak, I couldn't find a
reasonable solution.
We got a co-crystal dataset with the magic triangle I3C, extends to around
2.5A, this is the best data we got so far.
shelxc gives me the following result:
Resl. Inf. 12.59 7.75 5.84 4.77 4.08 3.59 3.22 2.94 2.70 2.51
2.35
N(data) 79 257 429 635 811 1010 1235 1384 1698 1555 1954
Chi-sq 0.69 0.63 0.60 0.67 0.61 0.88 1.05 1.02 0.80 0.55 0.42
79.4 34.1 30.3 32.2 31.8 26.6 21.1 14.6 8.3 3.9 2.4
%Complete 94.0 98.1 97.5 99.5 99.4 99.0 99.7 100.0 99.9 85.4 96.1
Multipl. 4.0 4.5 3.9 4.5 4.7 4.2 4.4 4.8 5.0 4.3 4.4
R(pim)% 2.27 1.72 2.29 2.05 2.02 2.62 3.51 4.84 7.90 14.43 22.78
Ranom% 6.49 3.68 5.19 4.29 4.27 6.35 9.50 12.97 20.90 33.58 52.21
