I thought to ask for something that I believe you have already
implemented, but I'm not sure of the best tool to use.
I have a cryoEM map where I refine my "high resolution"
structure. I also have the 3D variability of this map that shows
several maps varying around the consensus high-res map. I want
to refine an ensemble (20) of structures, one for every 20 maps
around the consensus map.
Is there a tool in phenix to do this?
I could refine individually the high-res structure into each map
incrementally; since every map differs a little from the
original one, Real-space-refinement could move the structure a
little at a time. Then I could combine all the PDBs in an
ensemble?
A tool to refine variability would be very useful. Input could
be a PDB and an ensemble of maps, and output would be all the
PDBs combined?