Hi Mohamed,
I solved a structure at 1.5 A with Sulfur MAD. There were only 4 Sulfurs in
the sequence and the signal has very nice.
Pay attention S does not give high anomalous signal.
I suggest you collect a dataset at 7kev (around 2.0A) which is the
absorption edge of S. You must collect very high multiplicity around 30-40
times with low intensity beam not to burn the crystal. This at kappa 0 and
kappa 45. Then you could merge both datasets. I remember an important
thing, on the absorption edge we had to be careful on the harmonics and
make a nice setting on the beam to remove them. I suggest you do also a
collection at 14kev far away from absorption edge.
For my case only Autorickshaw found the Sulfurs and solve the problem of
phases.
Hope it helps,
Ivan
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On 27 July 2015 7:17:18 pm mohamed noor
Dear all
Would you have some tips on collecting datasets for S-SAD at a 'normal' beamline (tunable from 0.5-2.5 A)? How helpful/important is it to use mini kappa?
Thanks.
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