I recently collected data at SSRL. I used autoxds and multiscale to merge couple of datasets and hoped to see some anomalous signals. Now comes the questions.

1. if I use the default sigma(=2), then autoxds will report weak anomalous signals and leave the friedel flag off. If I lower sigma to 1.5, it will report strong anomalous signals.

2. when I used Xtriage under Phenix to check the data quality, it reported weak anomalous signal or at above 10A.

3. I used aimless (in CCP4) to merge data, it also reported weak anomalous signal.

Which result should I trust? By the way, how can I view/display the ***.anamplot file, which is apparently xmgr format file, but I can not display in CCP4i.

Thanks!

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Charles Chen

Research Associate

University of Pittsburgh School of Medicine

Department of Anesthesiology

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