Hi, We've had reasonable success with NMR ensembles. A couple of suggestions for things to try: 1. We don't usually find that it helps to include more than the first 5 or so members of the NMR ensemble. 2. Even if the sequence identity is 100%, NMR structures usually aren't as good as X-ray structures for molecular replacement models. So rather than letting Phaser choose an RMSD for the model from the sequence identity, I usually set the RMSD to 1.5A for NMR models. Let me know if you're still having trouble. Randy Read On Dec 3 2007, ming lu wrote:
Hi everyones
I am trying automr to solve the structure of my protein. It has three domains, each domain has NMR structure available. So the sequence identity of my search model is 100%. I tried use mutiple components as search models, but I had a hard time to obtain a interpretable solution. Any suggestions on the use of mutiple components as search models? or suggestions on how to use NMR structure as search models? Thanks for your help.
Ming