Hi there

I have a data of heterotrimer protein complex at 2.9A resolution.

One protein consists of two domains. I tried phaser as well as molrep which gives a solution with only one domain.

Rotation function and translation function were found to be fine for these solutions.

I tried to find missing domain of the protein after fixing one of the domain or the other partner proteins using whole part or various truncations of missing domain.

I also tried to find and build missing domain using Rosetta with the solutions of Molrep or Phaser as template.

But, there were no solutions, or the solutions are clashed with other domain or proteins.

Furthermore, R and Rfree is 30 and 40, respectively, and I could not reduce them further.

There was almost no electron density map in the empty space so that I could not model manually.

Plz guide me ,how can i look for the missing domain in the protein.

regards

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INTEKHAB ALAM
LABORATORY OF STRUCTURAL BIOINFORMATICS
KOREA UNIVERSITY, SEOUL