Hi Tim,
It looks like you’ll need to navigate a bit of a learning curve
using the available online resources (rather than me re-typing
everything here!). Here are some useful pointers:
1) Phenix Video Tutorials:
https://www.youtube.com/c/phenixtutorials
There are several relevant tutorials on model building.
2) Self-Guided Tutorials:
Phenix comes with built-in tutorials, including many focused on
model building. You’ll see examples of how to use them in the
video tutorials mentioned above.
3) Phenix Documentation:
https://phenix-online.org/documentation/
It provides plenty of detailed guidance and references.
4) Workshops for Hands-On Experience. Consider attending one of
upcoming workshops with Phenix experts:
- ACA Satellite Phenix Workshop (Summer 2025);
- Week-long Crystallography & Cryo-EM School in Madrid (Spring
2025);
- Mini Phenix Workshop at the West Coast Structural Biology
Meeting (Spring 2025);
...and I’m sure more will be coming in 2025.
Please reach out if you have specific questions that aren’t
covered in these resources.
Good luck!
Pavel
Hi all,
Can you please advise once we fit the map and open in coot. What is next step to do in coot. I tried refmac but that required ccp4 file which doesn't have. Please some one can help in elaborating and sharing any tutorial would be helpful. I'm new bie and first time I am trying model building. Any help would be really appreciated. Thank you so much
Best wishes
Tim
On Tue, 17 Dec 2024, 13:47 Oleg Sobolev, <[email protected]> wrote:
Hello Iram,
The general workflow for Cryo-EM is described here:
It looks like you are ready to refine your model, so the tool for it is phenix.real_space_refine:that also has a video tutorial.
Let us know if you have any questions.
Best regards,Oleg Sobolev.
_______________________________________________On Thu, Dec 12, 2024 at 5:54 PM iram aziz <[email protected]> wrote:
Hello All._______________________________________________I got a nice 2.45 Angstrom map from cryo em data. I checked handedness, map model fitting on the chimera and opened in the coot. It looks nice. Can you please give me suggestions about the Phenix refinement steps.ThanksIram
phenixbb mailing list -- [email protected]
To unsubscribe send an email to [email protected]
Unsubscribe: phenixbb-leave@%(host_name)s
phenixbb mailing list -- [email protected]
To unsubscribe send an email to [email protected]
Unsubscribe: phenixbb-leave@%(host_name)s
_______________________________________________ phenixbb mailing list -- [email protected] To unsubscribe send an email to [email protected] Unsubscribe: phenixbb-leave@%(host_name)s