From: Tom Terwilliger <[email protected]>
Sent: 18 November 2023 11:44 AM
To: Reza Khayat <[email protected]>
Cc: [email protected] <[email protected]>
Subject: [EXTERNAL] Re: [phenixbb] Question about phenix.resolve_cryo_em

Hi Reza,

If you use a model in density modification with resolve_cryo_em, it is necessary to create a multi-model ensemble with refinement in order to reduce model bias. For details on why this works and is necessary, see:

https://scripts.iucr.org/cgi-bin/paper?S205979832001061X

All the best,

Tom T

On Fri, Nov 17, 2023 at 4:00 PM Reza Khayat <[email protected]> wrote:

Hi,

Is it possible to turn off the model refinement procedure in phenix.resolve.cryo_em and go straight into density modification? My current model fits reasonably well. Also, Does the routine work well with nucleic acid (e.g. something like RNA from ribosome)?

Best wishes,

Reza

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Thomas C Terwilliger

Laboratory Fellow, Los Alamos National Laboratory

Senior Scientist, New Mexico Consortium

100 Entrada Dr, Los Alamos, NM 87544

Email: [email protected]

Tel: 505-431-0010