Nat,<br><br>I have been using the auto.ncs function in phenix, but I haven&#39;t really looked to see if they are properly defined myself.<br><br>Pavel,<br><br>At this point I have not tried using the ordered_solvent command in phenix yet to find waters.  I will try that real quick before I bother you to have a look at it.  I&#39;ll let you know the updated R&#39;s afterwards.<br>

<br>Gino,<br><br>It is interesting that you say that, because I after I lowered the symmetry to P21, and re-ran xtriage, it mentioned that I had pseudo-merohedral symmetry, but I didn&#39;t pursue that further. My beta-angle was also ~90.2 in this case, and the twinning operator is the same (h, -k, -l).<br>

<br>I will detwin and re-refine and see what happens,<br><br>Thanks everyone and I&#39;ll let you know what works,<br>Bret<br>