Re: [phenixbb] Different cell content than expected in Xtriage

Dear Bishal, The correctness of the solution is not only based on the cell content analysis. In the first run the program might have located only 2 molecules or there is only 2 molecules in the asu. What are the TFZ and RFZ scores? Have you looked at the map after getting this solution? Need to provide bit more details to get a clear picture. Please take a look at the following link. https://www.phenix-online.org/documentation/reference/mr_overview.html Jobi Sent from my iPhone

On 18 Aug 2015, at 9:02 pm, Singh, Bishal wrote:

Dear all, I am solving crystal structure of a protein of size 33.7kDa in space group P 43 21 2 at 2.25 Å resolution. Unit cells constants are 121.51 121.51 156.6 90 90 90.

The cell content analysis in Xtriage showed 1050 residues (~ 3X 33.7 kDa) per ASU with 50 % solvent content, whereas the molecular replacement in mr-phaser produced a dimer (2X 33.7 kDa). When we consider a dimer per ASU, it should have approximately 70 % solvent content. Please suggest me whether solution is right or not. I shall appreciate your opinions.

Kind regards, Bishal

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