Depending on the size of the molecule, perhaps more low resolution reflections might be needed (and less high resolution). Hopefully you have good low resolution completeness (no overloads, detector not too close, etc).
On May 4, 2016, at 12:10 AM, Sharan Karade
Hello everyone,
i am working on data set which has resolution of 2.8 A space group C2, the protein have 394 a a . when i estimate Mathews coefficient it shows four to five molecules in asymmetric unit. when i try to get self rotation function Map by default parameter (10A-3A and 25 radius of integration) i was not getting any peak at all 180, 90, 120, 60. Or radius of integration affect the results.
Can anybody please help me to sort out with this problem, welcome for suggestions, thanks in advance.
-- Sharan C/O Dr. J V Pratap Senior Research Fellow, CSIR-Central Drug Research Institute, Lucknow
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