Hi PC, I'm sorry for the trouble! I'm not quite clear at what point the error message came up, but I would recommend downloading a current version of PHENIX from the web site (go to the "nightly builds" list on the download site and get a recent one) because that error should be fixed! Once you complete phenix.autosol, then you should be able to run autobuild with the results from autosol and it will do what you want, combining the model with the experimental phases to yield an improved map and model. All the best, Tom T On Jul 12, 2009, at 11:19 AM, Pei-Chun Lin wrote:
Hi,
I am the beginner of crystallography and have a 3.5A map generated by MAD method using phenix GUI. I already traced and built 2/3 of my protein. I wish to use this partial model combined with SAD to generate a new map and see if the new map can help me to deal with the rest of the structure. Does it sound reasonable to you?
However, after running AutoSol. I got this error message:
Failed to carry out AutoSol_score_ha_solutions: [Errno 2] No such file or directory: 'AutoSol_run_1_/TEMP0/ phaser_mtz.hand.mtz'
What should I do now? Can't phenix automatically calculate it?
PC
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