Hi All, I am trying to evaluate whether or not secondary structure restraints are going to help me with the refinement I am working on. When I ran in 'auto' mode, I noticed that by default phenix specifies several a-helices as 3_10. So, I would like to use an edited list of secondary structure restraints. Can I specify this file in the GUI? (Like Joe Noel, I, too, have become soft). I looked in what I thought to be the obvious places and did not turn anything up. Thanks for any help, Luke -- Luke M. Rice Assistant Professor and Thomas O. Hicks Scholar in Medical Research Department of Biochemistry, ND10.300 UT Southwestern Medical Center 5323 Harry Hines Blvd. Dallas, TX 75390-8816 phone: (214) 645-5931 email: [email protected]