7 Mar
2016
7 Mar
'16
4:40 p.m.
Hi Miyatake, could you please send me (and copy to Nigel) the PDB file (and any ligand CIF files you use) and tell what bonds you are expecting to have and that brake as result of refinement. Thanks, Pavel On 3/7/16 03:40, miyatake wrote:
Dear all,
I'm trying to a mutant of eYFP, which has fluorescence group, CR2.
During the refinement, however, the bonds between the CR2 and other amino acids were cleaved even I added the cif file created by the ReadySet.
Would some one give me suggestions to solve the problem?
Miyatake
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