OK Tom, thanks a lot for the very quick reply! I'll try that.

But now I'd like to understand - why is the sequence file necessary at all? I thought the local sharpening approach just did the same as the global sharpening, only sliding a small box throughout the volume. It seems then that this is not the case?

Best wishes,


--
Ricardo Diogo Righetto

2018-01-18 21:53 GMT+01:00 Tom Terwilliger <tterwilliger@newmexicoconsortium.org>:
Hi Ricardo,

I'm sorry, yes you do have to supply a sequence file for local sharpening.  I will fix the documentation.  If you want you can just put in a dummy sequence and supply a value for "solvent_content=xxx" and that will accomplish the same thing.

Let me know if that doesn't do it!
all the best,
Tom T

On Thu, Jan 18, 2018 at 1:42 PM, Ricardo Righetto <ricardorighetto@gmail.com> wrote:
Hi,

I am trying to do local sharpening with phenix.auto_sharpen, with the following command:

phenix.auto_sharpen map.mrc resolution=4.0 local_sharpening=True

Then it runs the global sharpening first as expected, but when it gets to the local sharpening it crashes with the following error:

Sorry: Please specify a sequence file with seq_file=myseq.seq

Nowhere it says that I would need a .seq file in order to do local sharpening (global sharpening works fine). Any ideas?

Thanks,

--
Ricardo Diogo Righetto

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--
Thomas C Terwilliger
Laboratory Fellow, Los Alamos National Laboratory
Senior Scientist, New Mexico Consortium
Tel: 505-431-0033