Hi Fengwei,
The model has to be pretty good as it is used to evaluate the maps. I
would improve the model first, at least by refinement , then combine the
maps, then maybe iterate the whole process with a new model.
-Tom T
On Mon, May 3, 2021 at 14:19 Zheng, Fengwei
Dear Phenix developers:
Thank you for developing this so powerful and elegant software packages for our community!
I am a newbie in cryoEM, and recently I got a consensus map, and split it into two parts to do the further focused refinement, after that I want to combine them together, and then to build my model, I learned that this "combine focused maps" module in Phenix can achieve this, I read through this online tutorial ( https://www.phenix-online.org/documentation/reference/combine_focused_maps.h...), for the starting input files, I seem need to provide my initial/consensus map, two focused maps and a model, but I have a question about this provided model:
1st, need me to provide my final refined model (i.e. I need to finish my model building firstly according to my two focused maps), or I can use a homologue model for this combination?
2nd, if I use a homologue model that only can be roughly fitted into my consensus map, will this affect my final combination results?
Appreciate!
Best, Fengwei
-- Thomas C Terwilliger Laboratory Fellow, Los Alamos National Laboratory Senior Scientist, New Mexico Consortium 100 Entrada Dr, Los Alamos, NM 87544 Email: [email protected] Tel: 505-431-0010