Hi Morten, It would be useful to include the entire sequence in the sequence file so that the solvent content is calculated correctly, as you guess. As for the fragment files...it won't make any difference if you include this part or not because mr_rosetta only extends a short distance (10 residues) beyond the template. All the best, Tom T
Hi everybody,
I'm trying to phase a dimer of a heterodimer of a large, ~700aa, and small, ~150aa, component. The only good search model according to HHpred covers the first 500aa of the large component. So I am wondering whether I should add the 150aa to the end of the sequence file and add them to the fragment files in order for phenix.mr_rosetta to do density modification correctly? Or does this not matter at all? According to HHpred and others the last 200aa are beta sheets, probably a a beta sandwich or two, and the 150aa component consists of helical elements but that is as close as it really gets.
Cheers, Morten _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb