![](https://secure.gravatar.com/avatar/557617dab68483788a909a14e9f994fb.jpg?s=120&d=mm&r=g)
9 Mar
2009
9 Mar
'09
4:27 p.m.
Greetings everyone: I have been dealing with a structure of a protein that has a Cys residue covalently modified with a peptide fluromethyl ketone. Can someone tell me how to include this ligand in phenix.refine? In the past, I have used CNS for this purpose. I created a topology and parameter file (using ProDrug server) and modified a patch in the refine script in CNS to establish a covalent connection between an atom in the ligand and gamma sulfur of the protein in question. Thanks. Chitta