Hi Reza,

Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions?

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Yes, this is somehow empirically determined.

I think that my predecessors of cryo_fit used to use 15 A, 10 A, 5 A resolution cryo-EM maps successively to avoid being trapped in local minima when they fit ribosome atomic structure into these.

Relevant references:

https://www.phenix-online.org/documentation/faqs/cryo_fit2_FAQ.html#how-can-i-provide-gaussian-filtered-maps

cascade mdff in https://elifesciences.org/articles/16105

best,

Doo Nam

From: <[email protected]> on behalf of Reza Khayat <[email protected]>
Date: Tuesday, February 9, 2021 at 11:51 AM
To: "[email protected]" <[email protected]>
Subject: [phenixbb] Cryo_fit

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Hi,

The gaussian filter used to prepare cryo-EM maps for cryo_fit is equivalent to a low pass filter. Can anyone suggest a resolution to low pass filter the map to? Or is this value empirically determined by screening through a number of resolutions? Thanks.

Best wishes,

Reza

Reza Khayat, PhD

Associate Professor 

City College of New York

Department of Chemistry and Biochemistry

New York, NY 10031