Dear PhenixBBers,<br><br>I&#39;m working on a 1.45 A structure I solved using MR (phaser) and I&#39;m pretty close to finishing, just plopping in waters and fixing rotamers. Rw = 19.8 Rfree= 22.8. I am a little concerned because my Wilson B is 27.00 while my average B for macromolecule is more like 43, and for solvent is 48. I have enough data to use anisotropic ADP refinement, which was a big help in bringing down the Rfree, but the average B hasn&#39;t really moved much. Should I be concerned about this? Should I try adjusting the wxu, or some other parameter? <br>

<br>Thanks!<br>-- <br>Geoffrey K. Feld<br><br>Department of Chemistry<br>492 Stanley Hall<br>University of California, Berkeley<br><br>&quot;Vigilia pretium libertatis&quot;<br>